Nucleotide Sequence of a cDNA Clone Encoding Ubiquitous [beta]-Amylase in Rye (Secale cereale L.)
نویسندگان
چکیده
منابع مشابه
Towards a whole-genome sequence for rye (Secale cereale L.).
We report on a whole-genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole-genome shotgun sequencing of the 7.9-Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total l...
متن کاملMolecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c.
We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,018 nucleotides and includes an open reading frame encoding a polypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase ac...
متن کاملPhysical mapping of a low-copy DNA sequence in rye (Secale cereale L.).
A 900-base-pair (bp) sequence from a cDNA clone of the rye endosperm-storage-protein gene Sec-1 was labeled with biotin and hybridized to Secale cereale 'Blanco' chromosomes. Hybridization was seen on the satellite part of the short arm of chromosome 1R, where the Sec-1 gene has been genetically mapped, in approximately 8.5% of the cells analyzed. The clone cross-hybridized at a lower frequency...
متن کاملTrace Elements Species Fractionation in Rye Flour and Rye (Secale cereale L.) Seedlings
Polák J., Mestek O., Koplík R., Šantrůček J., Komínková J., Kodíček M. (2009): Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings. Czech J. Food Sci., 27: 39–48. The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP−MS method....
متن کاملQTL mapping for benzoxazinoid content, preharvest sprouting, α-amylase activity, and leaf rust resistance in rye (Secale cereale L.)
Mapping population of recombinant inbred lines (RILs) representing 541 × Ot1-3 cross exhibited wide variations of benzoxazinoid (BX) content in leaves and roots, brown rust resistance, α-amylase activity in the grain, and resistance to preharvest sprouting. QTL mapping of major BX species using a DArT-based map revealed a complex genetic architecture underlying the production of these main seco...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1993
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.102.1.315